ea0081ep860 | Reproductive and Developmental Endocrinology | ECE2022
Magagnoli Matteo
, Mezzullo Marco
, Limoncella Silvia
, Tommasini Alessia
, Santi Daniele
, Pelusi Carla
, Simoni Manuela
, Pagotto Uberto
, Casarini Livio
, Fanelli Flaminia
Background: The classical steroidogenic route to the synthesis of dihydrotestosterone in Leydig cells involves delta-5 and delta-4 precursors. Besides, the backdoor pathway, encompassing progesterone metabolites, is gaining increasing interest in fetal development and men pathophysiology. Nowadays, liquid chromatography/tandem mass spectrometry (LC-MS/MS) is the ideal technique to simultaneously quantify large panels of steroids with elevated sensitivity and specif...